Dear Pallavi Kumari, the question is a subject of a wide spectrum of debate and opinions. I advise you to search within RG for similar questions. Please check the following links. My Regards

https://www.researchgate.net/post/How_to_determine_the_drug_loading_content_into_a_micelle

https://link.springer.com/article/10.1007/s11095-020-2783-4

https://doi.org/10.1002/jps.21342

If I were you, I should study the Langmuir and fruendlishisotherm to evaluate we qemax

Dear Pallavi Kumari, the question is a subject of a wide spectrum of debate and opinions. I advise you to search within RG for similar questions. Please check the following links. My Regards

https://www.researchgate.net/post/How_to_determine_the_drug_loading_content_into_a_micelle

https://link.springer.com/article/10.1007/s11095-020-2783-4

https://doi.org/10.1002/jps.21342

Dear Pallavi

We usually use spectrophotometric method to determine the loaded drug on a support. You have to check the concentration of the drug in the solution before loading and after loading to see how much of it is lost.The difference is the amount of loaded drug.

Unfortunately, it is practically impossible to calculate it theoretically as it depends on nature of a drug, nanoparticle and its components including shell, solvent, temperature and inevitably, on physico-chemical interactions (including parameters of solubility) of all components. But as Mohammad Kooti said it can be done through experimental techniques. I am sorry to disappoint you:)

Hi;

We can use Infra-Red spectroscopy. Please check the following documents.

With my best regards