Dear all,
I am staining two adjacent cellular structures solids with fluorescent antibodies (Alexa-488 and 555). I want to calculate the coverage of the small solid respect to the big one.
In disease state, they both reduce their volume although is more dramatic in the small one.
I know volume ratio is not enough nor surface area.
Could you suggest me any help?
Best regards,
Alan
Katherine Degen
How are you imaging this? Could you provide some more information on the statement volume ratio is not enough nor surface area?
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Ammar Alhaaik
You can use any microscope camera provided with analysis software. after you capture the cellular structure you can use calculate surface area of any structure by using this software .....personally I use scopeImage software.
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