I am confused about how to calculate the Reynolds Number (Re) of the largest eddies in a turbulent flow. It seems that many discussions I read (university course notes, for example) state that the characteristic velocity of the largest scale eddies is the fluctuating velocity and it should be used to calculate the Re for the largest eddies. However, course notes by Moshe Matalon at the University of Illinois at Urbana-Champaign state on page 4:

"A characteristic of a turbulent flow is the existence of a wide range of length scales, or eddy sizes. The largest size L, is the macroscale or the flow scale. Eddies of size L are characterized by the mean flow velocity U. The Reynolds number of these eddies Re = UL/v is large so that the effect of viscosity is negligibly small."

https://cefrc.princeton.edu/sites/g/files/toruqf1071/files/Files/2013%20Lecture%20Notes/Matalon/Notes-Lecture-14.pdf

I am trying to determine the Reynolds number exactly for the purpose of correlating to the range of scales for a microscale applicable to my system. For example the ratio of the Kolmogorov microscale to the largest eddy is equal to the Re to the -0.75 power.

It turns out that in my system the mean flow velocity seems to provide a good Re correlation (R2 = .91). If I use the fluctuating velocity, I get a significantly poorer correlation. My concern is how to explain this in a paper, since it seems that most believe the fluctuating velocity and not mean flow velocity should be used to calculate the large eddy Re.

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