Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Which solution do you use for low level actinide decontamination of laboratory surfaces like fume cupboards, work tops or floors?

Efficient decontamination of actinides like americium, uranium, neptunium and plutonium from laboratory surfaces. The decontamination should be easy in the sense that it could be performed on a...

03 June 2020 8,707 5 View

What is the best way to organize your MSD V-PLEX Human Proinflammatory Panel 1 raw data for analysis?

Hello People, does anyone have experience with the raw data analyses of the MSD V-PLEX Human Proinflammatory Panel 1? A friend of mine ran 4 plates and struggles with organizing the raw data for...

01 April 2020 3,566 2 View

How to do a non-linear regression analysis with multiple variables and a strong confounding variable?

Hi, I did outgrowth assays on spiral ganglion neurons from the inner ear with each supplemented by different concentrations of two neurotrophins. For each explant I did an image analysis and...

29 March 2020 8,703 7 View

Would somebody have suggestions for bioinformatic tools that predict changes in enzymatic (e.g. kinase) function based on changes in AA sequence?

Prediction of enzymatic activity based on common genetic variation.

19 February 2020 6,582 2 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How to compare diagnostic efficacy of several methods in a within-subjects design?

Hi, I conducted a study on faking in personality tests, where the participants received two instructions (respond honestly vs. fake good) in a within-subjects design (oder was counter-balanced...

09 January 2020 5,182 3 View

Is there not a common root of LBM and SPH modelling incompressible fluid flows?

Is there not a common root of Lattice Boltzmann Methods (LBM) and Smoothed-particle Hydrodynamics (SPH) modelling incompressible fluid flows? I see common grounds by means of a mesocopic...

30 December 2019 3,550 7 View

Why do I get a different results (load-displacement-curve) when applying different loads in ABAQUS/Explicit?

I'm currently modelling a steel-concrete composite construction with headed studs in ABAQUS/Explicit and want to analyse the load-displacement behaviour of the headed stud. In the first case I...

18 December 2019 3,070 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View