There is a big difference between particle size and particle size distribution. For 1% standard error on the mean you'll need to examine 10000 particles. If you wish to have the x90 to 1 % SE then you'll need 10000 particles in the x90+ part of the distribution. You'll have to decide what is a primary particle, what is an aggregate, and what is an agglomerate...

BTW, everything under TEM is an artifact of sample preparation and particle selection.

Image J is freeware written by NIH in the US. Google is your friend: http://bfy.tw/Mgm1

JayaKumar G, I suggest starting with this nice overview, published about a decade ago in Langmuir: 10.1021/la801367j

For more practical approach, there are several topics on RG already opened, dealing with the same topic, having some valuable comments, e.g.: https://www.researchgate.net/post/how_to_calculate_particle_size_from_TEM_images

Use image J software to calculate the particle size from SEM and TEM images.

You can see these previous RG discussions to calculate the particle size: https://www.google.com/amp/s/www.researchgate.net/post/How_to_calculate_particles_size_of_material_from_its_SEM_images/amp

https://www.google.com/amp/s/www.researchgate.net/post/How_to_determine_average_particle_size_from_a_SEM_image/amp

Dear JayaKumar G,

Following are the softwares which can be used to calculate the particle size from SEM and TEM images:

01. ImageJ software:

You can use the ImageJ software package. It is free and contains many powerful tools, including analyzing the size and shape of particles or You could measure by hand, using a ruler on a printed image.

02. ZEN software:

ZEN software to estimate average size of nanoparticles.

03. OLYMPUS software:

The OLYMPUS Inspector Series provides accurate and reproducible particle size and distribution data in accordance with International Standards for quantifying residue on filters, advanced particle analysis and non-metallic inclusion rating and is customizable for internal company standards.

04. Zetasizer Nano ZS90 and S90:

The Zetasizer range provides both exceptionally high performance and entry level systems that incorporate combinations of a particle size analyzer, zeta potential analyzer, molecular weight analyzer, protein mobility and microrheology measurements.

I hope the information will help you.

With Best Wishes,

Samir G. Pandya

ImageJ software.

You can calculate it using some software like Image J or just take a print out of the figure and measure the length of scale bar that is provided on the bottom. it will be of some x cm. so calculate x cm equals y nm. Then, measure size of all nanoparticles, convert them into nm and then take average of all.

sir, I tried with the Image J free software but its not installed in system. If you possible give any other route to get it and i need to plot the histogram of particle size.

There is a big difference between particle size and particle size distribution. For 1% standard error on the mean you'll need to examine 10000 particles. If you wish to have the x90 to 1 % SE then you'll need 10000 particles in the x90+ part of the distribution. You'll have to decide what is a primary particle, what is an aggregate, and what is an agglomerate...

BTW, everything under TEM is an artifact of sample preparation and particle selection.

Image J is freeware written by NIH in the US. Google is your friend: http://bfy.tw/Mgm1

you can used le logiciel ImageJ or XT docu.

You can used software image j

It is recommended to use image J software package.

Download link: " https://imagej.nih.gov/ij/download.html"

By using Image J software

With software Image J