How to calculate the number of free amines by TNBSA assay?

Mohammad Nazmul Hasan @Mohammad-Nazmul-Hasan

03 March 2017 0 1K Report

I have absorbance values of free protamine ( after tnbsa assay) and protamine conjugated linker for different concentrations. Now, how can I calculate the conjugation number from concentration and absorbance value?

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