Mohammad Nazmul Hasan

5 Questions 5 Answers 0 Followers

Questions related from Mohammad Nazmul Hasan

Do any of you know the procedure for Antibody fragmentation by using spin column and pepsin(Pierce F(ab)2 preparation kit)?

I have a few confusions of using Pierce Fab2 preparation kit. Sometimes, the spin column doesn't look properly compacted after centrifuging( then I centrifuged with high speed more times and...

19 March 2020 8,218 3 View

Is it possible to break down the S-S bond ( making SH) of antibody Fc region but still keeping the two Fc heavy chain together?

I want to break down SS bonds in Fc region by DTT or other reducing agents but I want to get the Fc with both heavy chain together, is it possible? Generally, after breaking SS bond, both Fc heavy...

07 November 2019 2,294 0 View

Hello, does anyone working with antibody fragments (Fab and Fc) conjugation or knows the chemical structure/functional groups related to conjugation?

Hello, does anyone working with antibody fragments (Fab and Fc) conjugation or knows the chemical structure/functional groups related to conjugation? If yes,kindly let me know, I have a query....

23 July 2019 8,366 4 View

Is tryptophan a problem in performing Ellman's assay for free thiol group determination?

In Ellman's assay protocol,it says -determining single cystein and lacking Tryptophan. Does it mean, having tryptophan in the sequence will not give a correct result ? And what's the correct...

10 March 2017 2,662 3 View

How to calculate the number of free amines by TNBSA assay?

I have absorbance values of free protamine ( after tnbsa assay) and protamine conjugated linker for different concentrations. Now, how can I calculate the conjugation number from concentration and...

03 March 2017 1,308 0 View