Hello and thank you for your interest in my question!
I am conducting experiments using Porcine Blood as an analog for Human Blood in the observation of the blood's physical coagulation properties. I am interested purely in the physical and fluid dynamic components of the blood.
The Porcine Blood needs to be collected from a farm and it needs to be doped with an anticoagulant (Heparin sodium salt from porcine intestinal mucosa) to keep it from clotting on the way to the lab. To counteract the Heparin, at the lab, I am using Protamine Sulfate to restore the blood's coagulation properties. I use a prothrombin meter (measures clotting time) to titrate the protamine and check that the blood has regained its clotting ability within an acceptable range of clotting times.
What I am hoping to replicate is how human blood clots when it is expelled from a vein either into a medical tube or into a body cavity.
I know I am being a bit vague in some areas, but can anyone see any major flaws with this model?
Erythrocyte aggregation levels in pig, horse, sheep, and calf blood samples were investigated and compared to that of normal human blood. The aggregation kinetics and adhesive forces between red cells, and an index of structure of the aggregates were determined with an erythroaggregameter (Regulest, France) at constant hematocrit (0.40 1/1) and temperature (37 °C). The adhesive forces and the index of structure in pig blood were close to those of normal human blood. The results for horse blood showed a very high level of aggregation kinetics and adhesive forces between red cells. For sheep and calf blood, little erythrocyte aggregation was found. To simulate different levels of red cell hyperaggregation in humans, a volume of horse plasma was replaced by isotonic NaCl in different proportions (5 to 40% V/V). The kinetics of rouleaux formation and especially the adhesive forces between erythrocytes were systematically decreased, while the index of structure was raised with increasing concentrations of isotonic NaCl. By replacing the porcine plasma with isotonic NaCl, normal and hypoaggregating levels of human red cells were simulated. The aggregation kinetics and the adhesive forces were reduced and the index of structure was raised when the concentration of isotonic NaCl was increased. In summary, large differences in the aggregation parameters were found between mammals. This study also showed that different human erythrocyte aggregation levels can be simulated by diluting the concentration of plasma proteins in equine and porcine bloods.
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