How to calculate the efficiency of plating(EOP) in bacteriophage host range tests?

More Sajith Dananjaya's questions See All

What is the the best source to extract Mycosporine-like amino acids in high yield?

We used marine micro algae

02 March 2019 2,184 3 View

Can chitosan metal (Ag and Au) nano composite encapsulate DNA?

I have done DNA encapsulation using chitosan nano particle. Also I found many referee for that. But i couldn't find any references for encapsulation of DNA with chitosan metal nano composite.

09 October 2017 6,195 3 View

How to explain ZnO NPs XRD peaks shift (lower and higher angle) with Ni doping?

I got the XRD pattern for undoped and 6% and 9 % Ni doped ZnO NPs. It is clear that there is no NiO peak and not change the crustal structure with Ni doping. But, 6 % Ni doped ZnO...

08 September 2016 4,349 7 View

Any body can please help me to explain the reason for the higher absorption of 9 % Ni doped ZnO NPs than 3% and 6% Ni doped ZnO NPs?

Undoped and Ni-doped (3%, 6%. 9%, 12%) ZnO NPs were synthesized by the facile reflux method. The optical absorption properties were studied by UV-vis sectrum using same concentration.

08 September 2016 7,011 9 View

What is the best fungal RNA isolation kit for Fusarium oxysporum ?

We need to study expression of ergosterol biosynthesis genes in Fusarium oxysporum

04 May 2016 178 4 View

Why do damaged cells give high fluorescence intensity with rhodamine 123?

Control, MIC level and MFC level of AuNPs treated C. albicans cell were harvested after 6 h expose time. The cells were washed with PBS, incubated with 5 mg/L rhodamine 123 (Rho123,Sigma,USA)...

02 March 2016 9,652 2 View

Can someone help me to identify this SAED pattern for silver nanoparticle?

This SAED Pattern was taken by Cs-Corrected Scanning Transmission Electron Microscopy?

11 December 2015 2,268 7 View

Why are chitosan nanoparticles insoluble in acetic acid?

I prepared chitosan nano particle using following step Low Molecular weight Chitosan (Sigma) was dissolved at a concentration of 3 mg/ml in 0.5% acetic acid solution, then raised to pH 4.6 with...

11 December 2014 5,932 11 View

Any one can suggest the method for quantification of Ampicillin( antibiotic concentration) in solution?

I followed "Spectrophotometric method has been described for the determination of ampicillin" But it was fail.

01 January 1970 1,868 17 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Issam Jumaa Nasser

I'm a follower

Jai Ghosh

Actually this has to be determined by you. Every researcher sets their validation of EOP for different phages and their respective hosts.

Michael J. Benedik

Take the optimal host (or the highest value you have) and assume that is 100%. The other EOP's will be whatever percentage less than that.