What is the the best source to extract Mycosporine-like amino acids in high yield?

We used marine micro algae

02 March 2019 2,148 3 View

Can chitosan metal (Ag and Au) nano composite encapsulate DNA?

I have done DNA encapsulation using chitosan nano particle. Also I found many referee for that. But i couldn't find any references for encapsulation of DNA with chitosan metal nano composite.

09 October 2017 6,143 3 View

How to explain ZnO NPs XRD peaks shift (lower and higher angle) with Ni doping?

08 September 2016 4,200 7 View

Any body can please help me to explain the reason for the higher absorption of 9 % Ni doped ZnO NPs than 3% and 6% Ni doped ZnO NPs?

08 September 2016 6,972 9 View

What is the best fungal RNA isolation kit for Fusarium oxysporum ?

04 May 2016 128 4 View

Why do damaged cells give high fluorescence intensity with rhodamine 123?

02 March 2016 9,606 2 View

Can someone help me to identify this SAED pattern for silver nanoparticle?

11 December 2015 2,213 7 View

Why are chitosan nanoparticles insoluble in acetic acid?

I prepared chitosan nano particle using following step Low Molecular weight Chitosan (Sigma) was dissolved at a concentration of 3 mg/ml in 0.5% acetic acid solution, then raised to pH 4.6 with...

11 December 2014 5,869 11 View

Any one can suggest the method for quantification of Ampicillin( antibiotic concentration) in solution?

01 January 1970 1,802 17 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View