I followed "Spectrophotometric method has been described for the determination of ampicillin" But it was fail.
Dear Sir. Concerning your issue about the method for quantification of Ampicillin( antibiotic concentration) in solution. Antibiotics are the chemotherapeutic agents that kill or inhibit the pathogenic microorganisms. Resistance of microorganism to antibiotics is a growing problem around the world due to indiscriminate and irrational use of antibiotics. Microbiological assay and high performance liquid chromatography (HPLC) method are used to quantify the potency of antibiotics. HPLC method is commonly used for the quantification of potency of antibiotics, but unable to determine the bioactivity; whereas microbiological assay estimates both potency and bioactivity of antibiotics. HPLC is a chromatographic technique used for the identification, quantification and purification of individual components of a mixture in analytical chemistry. HPLC is used extensively throughout the pharmaceutical industries for the quantification of antibiotics in pharmaceutical preparations. It is used to provide information on the composition of drug related samples. The information obtained may be qualitative, indicating what compounds are present in the sample, or quantitative, providing the actual amounts of compounds in the sample. HPLC is used at all the different stages in the creation of a new drug, and is also used routinely during drug manufacturing. It is more attractive than the classical bioassay in terms of speed, accuracy and precision. Hence, it has largely replaced the microbiological assays to determine the antibiotic concentrations in body fluids and pharmaceutical preparations. I think the following below links and the attached file may help you in your analysis:
http://www.sciencedirect.com/science/article/pii/S2095177916300521
http://onlinelibrary.wiley.com/doi/10.1111/1469-0691.12074/pdf
http://pubs.rsc.org/-/content/articlelanding/2013/ay/c3ay40847g#!divAbstract
Thanks
Lou et al (1997) described a HPLC method with fluorescence detection which may be useful.
This article may help
Selection of appropriate analytical tools to determine the potency and bioactivity of antibiotics and antibiotic resistance.2016.Journal of Pharmaceutical Analysis.
Hi: I think there are a ELISA kit for ampicillin, you can use it to quantification and quality use.
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Hi Sajith
You read some reference in attachment 1
https://www.sciencedirect.com/science/article/pii/S1878535212001013
4
https://www.jstage.jst.go.jp/article/cpb/61/9/61_c13-00197/_html/-char/ja/
8
https://www.ssjournals.com/index.php/ijasr/article/download/2061/1759
9
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwjEhd3Q8p_TAhXKcRQKHdeSDfkQFghbMAg&url=http%3A%2F%2Fciteseerx.ist.psu.edu%2Fviewdoc%2Fdownload%3Fdoi%3D10.1.1.921.4218%26rep%3Drep1%26type%3Dpdf&usg=AFQjCNGegkRMvUSly_dqsCvEnOHClZK-Cg&sig2=Q70LQ2AhIyCD0QT2t0JtWg
10
http://austinpublishinggroup.com/analytical-pharmaceutical-chemistry/download.php?file=fulltext/ajapc-v2-id1050.pdf
There are different methods: spectrophotometric, immunoassay and chromatography. I attach the different papers.
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