01 January 1970 4 6K Report

Such as in the image.

More Basma Elhariry's questions See All
I need a questionnaire on sustainable innovation appropriate for universities, Any Help?

Measuring sustainable innovation in Universities

11 October 2020 8,426 12 View

Difference between Caliberator and Normalizer?

Just wanna make sure i understand right.

24 July 2020 5,266 4 View

Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

11 September 2019 3,408 3 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

24 March 2019 4,805 3 View

Hi i have a problem iam trying to make a new experiment study in qpcr to analyze all my plates by stepone machine ?

e but obviously i cant add the ALL PLATES as one of them was run at 10 mins holding time then after 7 mins the machine stopped due to emergency then i opened it again after a min and iu sat the...

18 January 2019 4,732 2 View

Do we always calculate Relative Quantification in Qpcr as 2 power - deltadeltact?

I am using stepone PLus machine and in excel sheet attached to my plate . i found effiency mentioned 1? what does this mean? iam quiet new to the device

05 January 2019 2,708 2 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

11 December 2018 2,301 2 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

22 November 2018 7,890 1 View

Can i multiplex 2 genes and singplex the rest in same run and compare results?

i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...

20 November 2018 8,776 3 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

12 November 2018 7,416 5 View

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How to threshold an image despite bright lighting, using ImageJ/Fiji?

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