How to calculate recovery rate in HPLC analysis of methylene blue dye ?

15 October 2020 0 9K Report

I am trying to degrade an aqueous solution of 10 ppm methylene blue dye by using Photosensitizer chlorophyll (50 mg into 100 ml of 10 ppm MB solution) under a visible light system.

My question is that how can I calculate the MB recovery rate when chlorophyll is added to the working solution of MB dye? I have used the HPLC system for the calibration curve and its detection.

I want to see the effect of the matrices (chlorophyll) and this spiking recoveries rate?

I read some analytical calculation but getting confused, Please help me out,

Thank you !

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