I am recently applying the adoptive transfer method of naive T cells in young host. I wonder if someone can help me to calculate to % of T cell recovery of 2 months transfer. I am not sure the method to calculate that? is it the % of cd45.2+ T cells after transfer?
thanks
Hi dear Achouak Achour
I think the following paper can help you;
Best and kind regards.
http://www.jbc.org/content/282/9/6106.full
Article Transfer of CD8(+) T Cell Memory Using Bcl-2 as a Marker
It depends if you recover them from spleen, lymph nodes, how many lymph nodes you are harvesing. I personnaly prefer to use the absolute counts. If you inject 500 000 T cells, normally you recover a small fraction of what you injected. In this type of experiment, controls are essential.
Good luck
M
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