Does anyone know a specific marker of T cells in thymus?

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Achouak Achour

I want to specify that i worked on mouse

Nicole Acuff

Hi Achouak,

I do not know a specific marker for thymocytes, but you can inject mice with a fluorescent antibody (ie. CD45 FITC) i.v. for 3 minutes. 3 minutes is enough time for the antibody to stain cells in the circulation and not the tissue resident cells. You could then exclude the CD45 FITC + cells from your sort.

Good Luck!