Plotting a growth curve of bacteria when treated and untreated with a growth enhancer compound by measuring the increasing absorbance and fluorescence (Alamar Blue) as well. I want to calculate and plot the Ratio Generation Time. Anybody, kindly help me.
Regards
I can help you
you can use pour plate method and Sepecterophotometer
Hi Patrick,
Thanks a lot and a very happy and prosperous new year.
I think "Generation time" and "Ratio Generation Time" are not the same. Although, I may be wrong, kindly check an article @ pubmed with PMID: 10919401. If you feel that I am not understanding the concept, kindly help me. Anyways, I will definitely try what you have suggested.
Regards,
Hi Alaa Kareem,
Thanks a lot and a very happy and prosperous new year. Yeah I am using those.
Regards
use replicate cultures of each treatment, say five. can be small, test tubes. plot lnN (natural log of numbers or absorbance) vs time. Fit regression lines for each replicate on the linear parts of the curve (avoids physiological lag and starvation mode changes). Use a program that will estimate the slope (u; mu) and variance of the slope. Use the slope and variance estimates for each as means in a multiple range test to find significant differences. If you want the generation time, divide the slope values by 0.693 (natural log of 2)
u=(lnN0-lnNt)/t
TD=0.693/u
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