I have synthesized Polyelectrolyte complex of Chitosan (75% DDA) with SDS, and now want to know the molecular weight of monomeric unit of chitosan SDS complex for further chemical modification.
For chitosan SDS complex synthesis i have taken 161 as the molecular weight of monomeric unit of chitosan and the chitosan SDS complex formed in 1:1 stoichiometry.
How i proceed now with Chitosan SDS complex for further modification????
Hi add 18 to the mw of your protein thats your result. Like if your protein is 50Kda then 50+18
To calculate the ratio in the complex, you need to know the concentration of ionogenic units in the original purchased chitosan. For this you need to know the degree of deacetylation, for example, 80%. If all this is done and you take the chitosan / SDS ratio of 1: 1, then it is easy to calculate the molar mass.
Suppose i put reaction of 1 gm of chitosan (80% DDA) with sodium lauryl sulphate and if reaction goes in 1:1 stoichiometry to the ionogenic amine group then theoretically what is the yield of formed complex. molecular weight of sodium lauryl sulphate is 288.372 g/mol .
The manufacturer of chitosan should indicate the molar mass of chitosan.
Molecular mass of chitosan is in range of 50000 - 190000 D.
I am asking about the Theoretical yield of complex which is formed due to interaction of chitosan and Sodium lauryl sulfate..
To calculate the theoretical yield of a substance in a reaction, it is necessary to know the exact molecular mass of the substances. Your chitosan has a molar mass that is widely distributed. You wrote it yourself. Therefore, the calculation does not make sense.
Theoretical calculation requires exact molecular masses. Otherwise one has to carryout the calculation for the lower and higher molecular masses separately.
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