Pulp density and dilution factors are the two main factors have to be considered...
ICP/AAS analysis will provide the Cu concentration in wt/vol (e.g. mg/L), first it has to be convenrted to wt/wt (e.g. mg/g).. For instance, if xx mg/L is your ICP result, then
Cu leached = (xx mg/L X dilution factor X volume of the leachant (or culture)) / grams of chalcopyrite
So, the result gives Cu leached per gram of sample (mg/g is the unit)..
And then the Cu recovery can be calculated as given below,
Cu recovery = (Cu leached per gram of chalcopyrite / Total Cu present per gram of Chalcopyrite) X 100.
It is important to know what type of reactor you are using: column, CSTR, or batch. The calculations may differ. In the case of a CSTR system, it is also necessary to look at the accumulation in the system to do a proper mass balance.
Danilo Borja @ I understand and consider Reactor types, parameters etc. affecting the bio-transformation ability of an microbe but taking all these things into account there should be a basic formula which gives an output in numerical form.
Kindly revert for any discussion or clarification of my post.