I was finished PM (500 microgram/ml) DTT (100 microMolar) Assay . i got OD at 412 nm from different minutes (0, 15, 30, 60). Can anyone tell me , How to calculate DTT Activity (nmol/min/microgram)?.

0 Min = Control - 0.000,

S1- 0.024

P1- 0.022

T1- 0.022

E1-0.043

H1- 0.068

15 Min = Control-0.000

S1-0.021

P1-0.019

T1-0.016

E1-0.037

H1-0.015

30 Min = Control -0.000

S1-0.010

P1-0.009

T1-0.010

E1-0.022

H1-0.006

60 Min = Control - 0.000

S1- 0.011

P1- 0.010

T1- 0.014

E1- 0.032

H1- 0.009