Dr. Sankareswaran Senthil Kumar

6 Questions 22 Answers 0 Followers

Questions related from Dr. Sankareswaran Senthil Kumar

How to detect Cytokine and Chemokine in treated cell culture supernatant?

My Dear Friends, 1). I have A549 cell PM10 treated supernatant, i have to measure IL-1B, IL-6, IL-8 and TNF alpha influence after treatment. So i need protocol for this detection method without...

20 January 2015 5,185 6 View

Is a 0.22µ filter essential in-between Co2 cylinder and Co2 incubator connection?

In our lab, Sanyo-MCO-175 water jacket Co2 incubator was used for cell culture maintenance. Past 3 month we were faced contamination problem in our cell lines (A549 and MCF7). So I was checked all...

18 August 2014 6,827 7 View

MTT assay related doubts?

I was used different concentrations (100, 50, 25, 12.5, 6.25, 3.125 µg/ml) of particulate matter (PM10) treated with A549 cell line for 24 hours, 48 hours. I was completed 24 hours reading at 570...

17 July 2013 1,407 12 View

Cytokines (IL 6, IL-1β, TNF-a) and Chemokine (MIP-2) Assay protocols related queries?

I analyzed particulate matter (PM) sample from Selam industrial area, Tamil Nadu, India. I added my particulate matter in various concentration to the A549 cells. I used 96 well plates. After 16...

16 July 2013 9,121 0 View

How much amount (in ng) of vector or insert required for restriction or ligation reaction?

Usually I use 20 microliters restriction for the reaction which contain 30-100 ng vector or insert. Is it correct or can I change my concentration? I also used 1:3 ratio (Vector/Insert) for the...

19 June 2013 7,487 4 View

My product size is 63 BP and 79 BP - is it possible to elute this size (Using invitrogen gel elution kit)?

I have 20 BP forward primer and 24 BP Reverse Primer and also my product size oligos (63 bp & 79bp). I used my primers to amplify my products. Finally I got my exact size of the products in gel...

17 June 2013 7,930 29 View