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Questions related from Dr. Sankareswaran Senthil Kumar
I have 240 proteins which have been used in cytoscape GeneMANIA and then I used BiNGo to get 9 different networks. I merged our network image by Cytoscape merger but it appeared in separate group...
26 January 2016 4,325 1 View
My Dear Friends, 1). I have A549 cell PM10 treated supernatant, i have to measure IL-1B, IL-6, IL-8 and TNF alpha influence after treatment. So i need protocol for this detection method without...
20 January 2015 5,227 6 View
In our lab, Sanyo-MCO-175 water jacket Co2 incubator was used for cell culture maintenance. Past 3 month we were faced contamination problem in our cell lines (A549 and MCF7). So I was checked all...
18 August 2014 6,873 7 View
I was finished PM (500 microgram/ml) DTT (100 microMolar) Assay . i got OD at 412 nm from different minutes (0, 15, 30, 60). Can anyone tell me , How to calculate DTT Activity...
07 August 2013 7,127 0 View
I was used different concentrations (100, 50, 25, 12.5, 6.25, 3.125 µg/ml) of particulate matter (PM10) treated with A549 cell line for 24 hours, 48 hours. I was completed 24 hours reading at 570...
17 July 2013 1,457 12 View
I analyzed particulate matter (PM) sample from Selam industrial area, Tamil Nadu, India. I added my particulate matter in various concentration to the A549 cells. I used 96 well plates. After 16...
16 July 2013 9,155 0 View
Usually I use 20 microliters restriction for the reaction which contain 30-100 ng vector or insert. Is it correct or can I change my concentration? I also used 1:3 ratio (Vector/Insert) for the...
19 June 2013 7,524 4 View
I have 20 BP forward primer and 24 BP Reverse Primer and also my product size oligos (63 bp & 79bp). I used my primers to amplify my products. Finally I got my exact size of the products in gel...
17 June 2013 7,987 29 View
