Dear Rahul,

Do you want to calculate the concentration of fluorophore or quencher ?

Calculation of concentration of fluorophore is a bit difficult task.

There is no direct relationship between concentration and fluorescence intensity.

Under very dilute conditions, fluorescence intensity is directly proportional to the concentration of fluorophore. But after certain concentration, the intensity may be inversely proportional or no particular relation is observed.

this is because of the internal quenching raised due to inner filter effect.

thanks

Rajkumar Bandi sir,

I want to calculate how the fluorescent component concentration in my solution.