The Biomass Expansion Factor (BEF) and the Root-to-Shoot Ratio (R) are variables used to quantify carbon stock in forests. They are often considered as constant or species/area specific values in most studies. BEF for different species are available in ICFRE publications (for different agro-ecological regions of our country). Carbon sequestration rate can be easily calculated from BEF by multiplying 0.5 correction factor to BEF in the unit tons/hectare/year.
Carbon sequestration rates is determined by estimating annual biomass accumulation (difference in biomass between first year and second year). For measuring the annual biomass, you can follow guidelines given by IPCC (2006) or Pearson et al (2007) depending upon your forest conditions. Already available allometric equations can be used to measure the biomass of trees while destructive harvesting can be adopted for herbs, shrubs and litter.