Hi everyone, I've been working antioxidant assays and α-Glucosidase (EC 3.2.1.20) inhibition assay with pomegranate peel and juice, using a methanolic extract of peel but not performing any extraction to the juice. With the extracts, everything works perfectly, IC50, and kinetics etc. But the juice seems to have so much sugar that under the assay conditions reacts with the enzyme before the pNPG does, giving high values of "inhibition" at low concentration that are not correlated with the actual antioxidant capacity. Is there anyone with experience with similar situations?
Kind regards and thanks in advance,
Fco.
I think this is challenging, I suggest that you perform dialysis first and then determine the glucosidase activity, but it is cumbersome and adds errors.
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