We will be collecting ovaries and surrounding tissues from slaughtered animals for oocyte collection. One of the goals is also to measure the diameter of arteries to ovary by ultrasound in a water bath. Do we need to ligate the vessels and fill them with fluid in order to be able to see them? Does anyone have suggestions/ a protocol for this?
Hi Victoria.
I think it is quite hard work to visualise vessels in ovaries which were removed from pelvis. However it should be impossible. In case you really want to measure the diameter of vessels in these ovaries you should fill them with fluid as you wrote. But be careful, the diameter can change compared to physiological status.
Have you considered another way of visualization? Maybe these works may help.
http://www.scielo.br/pdf/aabc/v89n3s0/0001-3765-aabc-201720150864.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891950/
https://www.nature.com/articles/srep38057.pdf
Good luck with your research.
Regards,
Jiri
