I have to stain roots to see arbuscular fungus inside their roots. I use the staining method of the title, but I always get a lot of bubbles inside the roots. Although the bubbles allow to see the hyphae and arbuscules, the pictures with lot of bubbles are not very suitable for publication.
Does anybody know how to avoid (or get rid of) thoses bubbles ?
Thanks
Hello,
You can try to keep the samples in low vacuum before staining. This can be done in a vacuum chamber, or simply with a syringe and a sealed bottle. Low vacuum will extinguish the bubbles without affecting tissue structure. The samples must obviously be immersed in some liquid,
As Jordano I was also going to suggest applying vacuum. As a comment, I don't think that bubbles will prevent you from publishing the results. Even the original publication features bubbles.
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