Amperometry technique for Glucose Detection?

Hello All, I'm fabricating a simple glucose sensor using a platinum wire as working electrode coated with Glucose oxidase, BSA, glutaraldehyde and Nafion. I ran the CV against a platinum counter...

04 May 2019 6,873 7 View

How to convert imagej's fluorescence measurements in pixels to RFI?

Hello! How would we convert the pixels acquired from ImageJ for fluorescence intensity to relative fluorescence intensity? (RFI) Thank you.

31 December 2018 2,587 1 View

What is the possibly cheapest cell viability assay/cell attachment assay, and not on a 96 well plate?

Hi Fellow Researchers, I have cardiomyocytes plated on 6 well plates, on sensors. I need a cheap assay determining or confirming their attachment and viability. MTT can't be of use as any...

07 August 2018 7,046 5 View

How long after cell culture will cardiomyocytes beat?

Hello all I have cultured Rat Cardiomyocytes just regularly in a dish and on chips. I need to see their contractability and I was wondering if anyone is familiar with their post-culture process?...

10 November 2017 2,992 5 View

3D cell Culture protocol?

Hello All, I am trying to do a 3D cell culture for Smooth Muscle cells and Vascular Endothelial Cell lines. I found a lot of reviews and publications but I was wondering if anyone can refer me...

09 October 2017 1,299 5 View

Can anyone recommend a cheap antigen-antibody model?

Hey everyone, I'm looking to test a combination of two techniques and machines on antigen antibody interactions and antigen antibody is not my specialty. So can anyone please tell me what could be...

31 December 2016 7,104 6 View

What is a simple decent protocol for immobilization of MP-11 (microperoxidase 11) on SPR gold chips?

07 August 2016 5,797 7 View

How do I create the chronoamperometry staircase like graph that increases or decreases with concentration?

My graphs always look like picture 2, while I need something like picture 1. I've read a lot on chronoamp, but none explains how to relate the two graphs together. I need the current vs. time...

04 May 2016 9,328 12 View

Is calmodulin a voltage sensitive protein? Is it controllable using electrical properties?

Calmodulin is the protein I've chosen to work with and since I'm adding electrochemical SPR to screen its reaction with target proteins. I'm wondering from a biochemistry and organic chemistry...

11 December 2015 5,779 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View