In a project I collected samples from a metals rich environment of gold mine. Isolated the bacteria residing in metals contaminated environment from a long time, purified, characterized and sequenced whole genome and all the stuff related to categorise the strain. Problem is that, the strain that i got is similar to Rhodanobacter thiooxydans LC2 & 2 strains of Rhodanobacter denitrificans NCBI BLAST as 99%. On another very authentic site "ezbiocloud" where they only have type strains to compare, I got 97% with Rhodanobacter thiooxydans LCS and 2 strains of Rhodanobacter denitrificans and Rhodanobacter thiooxydans LC2. Funny thing is when i make the phylogenetic tree, it appears to be different from both. Strain is pure and I have 16S results all the annotations and whole genome sequence but still I am not sure which thing I should consider. and how I can assure the real strain. or if this strain is some what in between them then how can i confirm the thing.