I want assess the rate of intracellular parasite killing in side the infected macrophages in in vitro experiment. what method is opt for assess live or dead parasites inside the macrophages? Please help me..
You may use a vital stain such as acridine orange to stain the parasites, and other stain for the phagocytic cell. Acridine orange stains degraded DNA in orange and intact DNA in green. It is also useful to stain engulfed apoptotic cells and organelles such as lysosomes. Hope it helps!