prepare two reagents; one is B7-transfected fibroblasts and other is CTLA4-IgFc fusion protein. You can screen the inhibitory ability of your compound to the binding of CTLA4-IgFc to B7-fibrobalst.
Thank you very much for your response, appreciated. Can't we just use spleenocytes stimulated with SEB or so in presence of CTLA4-IgFc fusion protein? B7-is expressed on APCs and ready will be T cell proliferation? what do u say?