Dear Shamee

You need to give ionformation about the case and the results for other people to understand the problem and answer your query. Thank you,

In this case, we must test this antibody against an antigen of specific epitope.

Kindly mention complete scenario.

Does it involve in transfusion? Kindly, please mention and detail the case because its similar to antibody identificasion in immunology study.

Red cell antibodies can be detected with a direct Coombs test (antibody fix to red cells) and/or with an indirect Coombs test (free antibodies in serum or plasma). A positive  direct Coombs test means the patient have an autoantibody directed to his own red cells, that is an immune hemolytic disease (sometimes without hemolysis or anemia). If the patient have a positive indirect Coombs test with a negative direct Coombs you can pressume he or she is alloimmunized by transfusions. You need to have a pool of identified red cells for the most common antigens (there are also commercially available panel detectors of 2 or 3 tubes). Once done you may assume, with the antigen chart, to which system is or are the antibodie/s directed. Usually a polytransfused patient first have alloantibodies against the Rh system (most commonly anti-E), and then for the Kell system (most commonly anti-K1). To identify correctly the antibody you must test the patient's serum or plasma against a panel of at least 11 tipified red blood cells and ending with the antiglobulun phase (Coombs antiglobulin-serum). Remember that red cell antibodies may be cold or warm (reacting preferably at 4ºC or 37ºC respectively). Therefore the test should be done at both temperatures. Details of the techniques may be found at the AABB Manual.