What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

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Please recommend the optimal optical component and its parameters. Thanks a lot

21 December 2020 7,678 3 View

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02 December 2020 7,293 2 View

The titanium electrode corrodes during the ohmic heating process, will it affect the electrical conductivity of the sample?

The titanium electrode corrodes during the ohmic heating process, will it affect the electrical conductivity of the sample? Thank you .

19 November 2020 4,082 2 View

How to reduce background noise when testing cholesterol level in rat serum?

l've got rat serum samples with different background (from pink to yellow) and l'm trying to test total cholesterol, HDL and triglyceride in mice serum. l'm worried that the background noise...

14 November 2020 6,628 3 View

How to measure the focusing efficiency of Fresnel zone plate (FWHM is in micrometer scale) ?

We want to measure the focusing efficiency of the Fresnel zone plate with a very small FWHM. "The focusing efficiency is defined as the ratio of the total power in a circular aperture with a...

01 November 2020 1,252 7 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

How to use first-order markov model predicting continuous time-series data?

Hello, everyone, I want to use markov model to predict continuous time-series data, and I read some references, It seems that the model is more suitable for discrete data , so I divided the...

06 October 2020 8,239 3 View

Failure in using Gene Pulser Xcell (Biorad) ?

02 October 2020 8,642 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View