Yi Jiang

3 Questions 3 Answers 0 Followers

Questions related from Yi Jiang

Extracting granulocytes DNA, no white precipitate after RBCs lysis?

Hello, I am extracting DNA from granulocytes. I firstly extracted PBMCs from fresh blood 2 weeks ago by ficoll, and the packed granulocytes in the bottom was conserved in 4℃ till today. When I am...

04 May 2023 1,847 0 View

How can I apply blast to verify the specificity of a primer with degeneracy “I” ?

I found a pair of primers of my target gene in references, which is Staphylococcus-specific, as F 5’-GGCCGTGTTGAACGTGGTCAAATCA-3’ R 5’-TIACCATTTCAGTACCTTCTGGTAA-3’ I want to verify its...

06 April 2023 3,990 2 View

How to apply “Absolute quantification/fixed points methods ” in Taqman qPCR in Applied Biosystems ViiA ™ 7 Real-Time PCR System?

I know that in LighterCycler 480 system, we can use Absolute quantification/fixed points methods and then set a reasonable noise band to estimate the CT values. My workmate told me to use this...

02 February 2023 2,524 3 View