Hi all, is anyone skilled in Image J? I'm having a problem with image processing in doing my research.
I want to analyze the particle size distribution of the droplets in the image, but since the contrast between the droplets and the background is too small and only the boundary is clear, I don't know how to do binarization in Image J to analyze the particle size distribution. I would like to ask you how to analyze the particle size distribution in IImage J for this kind of image where the difference between droplet and background gray scale is small but the boundary of droplet is clear?
Hello, I think the boundary color can deliver a real advantage for analysis. I have measured muscle fiber areas that were having similar color with the background. Since the boundary was distinct therefore i could use ImageJ to analyze the area. Here is the link to the video. Hope it helps. https://www.youtube.com/watch?v=xeAuh_E8z8k
Hi Zundong Xizo !
I would suggest you have a look at the following video which seems to fit your needs quite well. It deals with segmenting and measuring the radious of touching circular objects from images:
https://www.youtube.com/watch?v=5SftMqAwNxE
All the best,
Máté
This is the kind of results you can expect. How many of the edge, incomplete blobs are included will be influenced by the parameters you use.
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