Hello, I conducted TEM on cells pre-treated with various compounds, which induced cell stress and death. I originally measured mitochondrial size, swelling of the endoplasmic reticulum, and vacuole presence. However, I read a publication that suggested a difference in vacuole membrane based on type of cell death induced. I re-reviewed my samples, but I'm unsure how to interpret the vacuoles. Some look just like tearing, without a membrane at all.
Any insight or resources appreciated
Mentioned pub: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10860/1086002/Photodynamic-therapy-and-paraptosis-an-update/10.1117/12.2506325.full?SSO=1
Double membranes (twin bilayers), are characteristic of autophagic death. Other than that you look for chromatin condensation status to see necrosis vs apoptosis, mitochondria fragmentation or ER aggregation often studded with ribosomes in calcium overload related death. Alternatively, you can refer my paper here
Article Blebbishields, the Emergency Program for Cancer Stem Cells: ...
Which has More TEM images in supplementary materials.
let me know if you have any questions.
J.