How to analyze SRB ( Sulforhodamine B ) assay results?

12 December 2018 1 7K Report

I am working on melanoma B16-F10 cell line and after treatment whit different concentration of drug I do SRB protocol then I read their absorb at wavelengths of 545 nm. now, how to analyze these results? What should be the range of control absorbency?

Lakshmi Mohan

You can use the following protocol to calculate IC 50 for your drug. PDF attached for your reference.

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