I want a protocol to determine amylose and amylopectin composition in rice..
You can extract and determine total starch by reducing sugar analysis using standard AOAC methods. Using a suitable spectrophotometer, amylose iodine complex can be determined at about 590 nm. Amylopectin can subsequently be determined by calculation. Check A.O.A.C. method.. Also check Williams VR.Wei-Ting Wu , Tsai, H.Y and Bates, H.G. 1958. Varietal differences in amylose content of rice starch. Journal of Agricultural and Food Chemistry.6 (1), pp 47–48.pubs.acs.org/doi/pdf/10.1021/jf60083a009. Methods that are based on near infra red transmittance spectroscopy are also available if you have the facilities.
You can perform size exclusion chromatography to determine the debranched chain-length distributions of amylose and amylopectin in rice varieties.
You can use Williard's non-interference equations to determine amylose and amylopectin rice powders using spectrophotometer.
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