how to analysis the phenolic compounds by TLC, the method and solvants?
Dear Laouini,
Please read the following:
1-Methods of analysis
TLC was performed with two different chromatographic systems. Thin layers were
prepared from microcrystalline cellulose and silica gel G. Standard solutions and samples were spotted on precoated plates of cellulose microcrystalline with fluorescent indicator and developed with mobile system of ethyl-acetate:formic acid:acetic acid:water (100:11:11:26, v/v/v/v). The other chromatographic system was silica gel GF254 as stationary and chloroform:ethyl-acetate:formic acid (5:4:1, v/v/v) as mobile phase. Substances were identified using UV detection at 254 nm. For visualization, plates were sprayed with FeCl3 (2% in ethanol).
http://www.doiserbia.nb.rs/img/doi/1450-7188/2004/1450-71880435199M.pdf
2-We report a new solvent system for the silica thin-layer chromatography of phenols, FEAP, comprised of formic acid, ethyl ether, and acetonitrile saturated with pentane. The solvent mixture runs rapidly and cleanly separates many closely related compounds and circumvents several problems related to chemical alterations of phenols analyzed by more conventional solvent mixtures. We have obtained the Rf values for 41 different phenols.
http://chromsci.oxfordjournals.org/content/29/11/476.abstract
3-Molecules 2013, 18, 2328-2375; doi:10.3390/molecules18022328
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Review
Techniques for Analysis of Plant Phenolic Compounds
Ali Khoddami 1
, Meredith A. Wilkes 1
and Thomas H. Roberts
This is a review article that sheds light on all methods used for phenols analysis, for more details, see attached file.
Hoping this will be helpful,
Rafik
Toluene : acetone (9:1) is good choice of mobile phase for class of phenolic compounds in preparative TLC plate .
Solvent system saturation must be 10 min.
You got reproducible RF (0.4) alignment of any phenolic class of compound.
Courtsey .Sthal chromatography.
Hi, maybe you can search in the TLC Camag Data base below , best regards
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
Toluene : Acetone (9:1) or
Ethyl acetate-formic acid-glacial acetic acid and water with a ratio (100:11:11:27)
Chloroform: ethyl acetate (60:40)
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts