Dear professors, i am confused with how to figure out the tea bag index?

Hello, i am confused with how to figure out the tea bag index,could you please tell me how to compute the letter "a" and "k". Can i use R or Sigmaplot to fix it?

09 December 2020 6,705 3 View

How to analyze a time series of temperature mean whether different or not?

I have 6 culture tank, 2 treatment with 3 replicate. I test temperature of every tank everyday, for 14 days. Now I want to compare whether this 2 treatment temperature have significant different...

06 December 2020 834 7 View

Strain Hardening Phenonmenon under Uniaxial Tension when using ABAQUS CDP?

Dear all, I encountered some problems when using the ABAQUS CDP model. Could you please give me some suggestions? I did a monotonic uniaxial test using a single 3D solid element (C3D8R) and the...

04 October 2020 7,111 3 View

Buckling Analysis - Risk vs Ex/Implicit vs Static Stabilised - Displacement vs Force Control ?

Dear all, I am studying how to conduct lateral-torsional buckling analysis of a simply supported beam. The beam is purely elastic, and the boundary conditions are shown below. I started with a...

21 July 2020 3,761 2 View

How do I detect the onset of each period of a noisy periodic signal (MATLAB preferred)?

I have a noisy periodic signal, like the attached picture, is there a way to extract the time of each onset of each period (shown by red arrows)? MATLAB preferred other programming languages also...

16 July 2020 5,988 8 View

Dose anyone knows 3AO cells (ovarian cancer cells ) background?

25 March 2019 9,796 1 View

Variable importance (a new R2 method)+ Could I just make use of the original model weights to predict direcly under the modified datasets?

When measuring the variable importance, Olden(2015) proposed a new R2-based measure of relative variable importance after revealing methodological weaknesses of PSW and PCW. Its basic steps is...

27 February 2019 988 1 View

In Q-Exactive Orbitrap or Q-TOF, how does the quadrupole select topN ions for MS/MS analysis in data-dependent acquisition?

In high-resolution tandem mass spectrometry such as Q-Exactive Orbitrap and Q-TOF, full scan/ddMS2 is commonly used. In this mode, a full scan is first acquired, followed by fragmentation of top N...

25 February 2019 6,737 6 View

Where can I get a open source code for Multi-spectral segmentation?

15 April 2018 684 4 View

How to synthesize 10nm Ag nanoparticles?

I follow the literature to synthesize Ag nanoparticles, but I can't get the same result. I think the problem is that when should adjust pH?

25 January 2018 9,486 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View