Dear Saurabh,

there are many genetic software that can be used for genetic diversity such as GenAIEx, Darwin, NTYSYS etc. Use any one which is comfortable to you.

For preparing the input file, see the manual provided by the developer of the software.

I think if you can use the darwin softwere, the SSR data shoulcbe classify as the alleleic data because the SSR is the codominan marker and if you choice the single data , It spesific to dominant marker. thank you.

darwin focus to dissimilarity and any softwere focus to similarity like NTYSYS

Hello,

have a look on previous RG posts on the same or very similar issue:

https://www.researchgate.net/post/Can_someone_get_me_a_software_that_I_can_use_to_analyze_SSR_data_for_diversity_study?_iepl%5BgeneralViewId%5D=SajNdW16WwNA3bERcyNhwUCBPCzeL0vzfaNq&_iepl%5Bcontexts%5D%5B0%5D=searchReact&_iepl%5BviewId%5D=XXKGymhicR76257bzP9URd0Mq0TkrxgCiSsO&_iepl%5BsearchType%5D=question&_iepl%5Bdata%5D%5BcountMoreThan20%5D=1&_iepl%5Bdata%5D%5BinteractedWithPosition9%5D=1&_iepl%5Bposition%5D=9&_iepl%5BrgKey%5D=PT%3A5b77a7e2b93ecd16c41b7a9a&_iepl%5BtargetEntityId%5D=Q%3A5b77a7e2b93ecd16c41b7a9a&_iepl%5BinteractionType%5D=questionView

Remember to check also for null alleles:

https://www.researchgate.net/post/Is_there_an_on_line_software_for_the_detection_of_null_alleles_on_SSR_data?_iepl%5BgeneralViewId%5D=SajNdW16WwNA3bERcyNhwUCBPCzeL0vzfaNq&_iepl%5Bcontexts%5D%5B0%5D=searchReact&_iepl%5BviewId%5D=XXKGymhicR76257bzP9URd0Mq0TkrxgCiSsO&_iepl%5BsearchType%5D=question&_iepl%5Bdata%5D%5BcountMoreThan20%5D=1&_iepl%5Bdata%5D%5BinteractedWithPosition20plus%5D=1&_iepl%5Bposition%5D=24&_iepl%5BrgKey%5D=PT%3A5b21541ee5d99ea70b49ac3a&_iepl%5BtargetEntityId%5D=Q%3A5b21541ee5d99ea70b49ac3a&_iepl%5BinteractionType%5D=questionView

Good luck

Piotr

Thank You all Umakanta Ngangkham , Piotr Androsiuk , Muhammad Fuad Anshori

  need to score to 0, 1 and used NTSYSpc

Thank you @ Khaled FM answer sir

You can use many variety of softwares for analysis. The analysis depends upon what you want. If your objective is look for diversity the Darwin, power marker are OK. If you want to infer population structure then you can use GenAlex, Areliquin, Structure, etc

You can use GenAIEx, Darwin, NT-SYS PCA, power marker etc.

There are many software for genetic diversity analysis like, STRUCTURE, Arlequin, POPGENE, GENPOP, AMOVA etc.

Thank You Tabasum Akhter

U can use popgene or genealyx for genetic diversity analysis, as mentioned by others Arlequin can also be used.