Sorting negative cells from GFP dim parental, please help?

I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...

23 September 2019 3,940 1 View

FACS-related Co-op project, can someone help me with their input?

Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...

18 September 2019 8,911 2 View

Substitutes for fill solution for a Hanna Instruments pHmeter?

I have a Hanna Instruments pHmeter whose electrode needs to be refilled. However, at the time I'm unable to get the fill solution from HI directly. In the specs of the equipment, it establishes...

03 February 2019 4,382 3 View

How to calculate . metabias for continuous data in Stata?

I am working on a meta-analysis of RCTs, and I have to calculate .metabias (several tests, including Egger's) for continuous data (variables such as means and standard deviations). What is the...

07 May 2017 9,761 2 View

How to calculate . metabias for continuous data in Stata?

I am working on a meta-analysis of RCTs, and I have to calculate .metabias (several tests, including Egger's) for continuous data (variables such as means and standard deviations). What is the...

04 May 2017 5,099 2 View

Which DNA extraction method do you suggest from formalin-fixed, paraffin-embedded tissue?

10 November 2015 1,109 4 View

Does anyone have ideas on how to estimate maximum dissipation power before a Al/Al2O3/Al _metal tunnel junction burns up?

My Al/Al2O3/Al junctions easily burns up, specially when I change dimensions. Can anyone give me a hint on how to estimate the maximum current or voltage that can go through without damaging them?

17 March 2015 7,051 3 View

Which would be a good protocol to stain Heat shock proteins that have been exposed to the cell surface?

11 December 2014 4,739 2 View

Can you recommend a model to perform regression with high dimension data?

My data-set has 23377 instances for training (7792 for testing). The dimension of the data is approximately 28000. Each instance represents a document and the target variable is the age of the...

08 September 2014 1,313 26 View

Face recognition: Holistic or feature based approach?

In the task of face recognition I aim to choose between two approaches in order to tackle the problem. First a holistic approach using eigenfaces (PCA) over 2D images and/or depth maps of 3D...

05 June 2014 5,094 8 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

How to choose the Hidden Layers Number and RBM Size in a DBN network?

Hello, When using a Deep Belief network (DBN) for images classification, how could we choose the number of hidden layers (number of Restricted Boltzmann Machines) and nodes (RBM size) in a hidden...

09 February 2021 4,618 5 View

Is there any similarity index between two time series?

Hi We have two time series dataset. For example mean daily temperature from two stations. Each of them have 30 data for a month. T1={ t1 , t2 ,... , t30 } T2={ t1 , t2 ,... , t30 } Now we want to...

06 February 2021 6,434 7 View

Matrix based scientific data analysis: extrapolating to matrices of sets?

Data sets, when structured, can be put in vector form (v(1)...v(n)), adding time dependency it's v(i, t) for i=1...n and t=1...T. Then we have a matrix of terms v(i, j)... Matrices are important,...

30 January 2021 9,295 6 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

How can I make unsupervised clustering of trees species using algorithms like HAC, Kmeans... in R?

I am working on the development of multi-species volume allometric equation from diameter (DBH). I would like to form groups of species with the largest possible volume-diameter correlations by...

19 January 2021 5,752 5 View

In which case meta-heuristics algorithms cannot minimize the network error?

Hello, I am working on hybrid neural network. I have combined ANN with two different evolutionnary algorithms to build a prediction model of 6 inputs and one output with one hidden layer of 7...

18 January 2021 7,144 3 View

Is It useful to use text Pre-processing techniques (Stop Words Removal, Stemming) in the sentiment analysis classification?

Hello, I read some papers they used Pre-processing steps with text that will classify based on Sentiment Analysis. My question is, can I use text Pre-processing techniques in the sentiment...

11 January 2021 5,932 7 View

What is your philosophy about life ?

how do you understand life as it is really big suspense, why we are here, our consciousness is big unconsciousness and our unconsciousness big consciousness.

25 December 2020 7,632 19 View

Do you think knowledge should be in equal access to everyone ?

everyone knows the importance of knowledge but knowing this too why there is a restriction in getting access to it what is reason behind this.

23 December 2020 2,934 6 View