How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

How to extract RNA from pancreas?

RNA extraction from pancreas of ruminants is very difficult due to the existence of abundant RNase. Previously, our research group tried to remove the pancreas from animal immediatley after...

20 September 2020 1,097 2 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

How to seprate the grid cells of landsldie source areas using ArcGIS in batches?

The source areas of landslides are useful for accurately mapping the susceptibility of landsldies. However, an inventory of landslides always contains the source and deposit areas of the...

05 March 2020 5,582 3 View

Roche has stopped its Universal Probe primer design service, does anyone know where or how we can design primers to fit Universal Probe Library?

Roche announced the Universal Probe primer design service will be stop in the end of 2020, but the primer design website has already been shutted...

10 February 2020 4,745 2 View

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ?

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ? Hello everyone I have a question while using ziptip Do you suggest that reuse the zip after one sample binding and...

11 December 2019 7,865 3 View

What reason can affect drug concentration to cell viability in MTT assay ?

I did MTT to find the best drug(for cancer) concentration to do Western blot, as drug concentration got higher cell viability got lower, but at the highest concentration has a peak on cell...

14 August 2019 3,745 5 View

Does mesoporous silica NP dissolve in water?

Hi, guys. Recently, I was doing research on mesoporous silica. I used the traditional ingredient to synthesis it, namely TEOS, CTAB, ammounia aqueous, ethanol and water. To my surprise, when I...

08 July 2019 9,961 5 View

What kind of matrigel for orthotopic injection of breast cancer cells into the mammary fat pad of mice?

I am planning to do orthotopic injection of breast cancer cells (MDA-MB-231 and 4T1 cells) into the mammary fat pad of mice to observe cancer metastasis and tumor growth. In lots of references I...

04 July 2019 6,278 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How reliable are degrees of freedom for lme mixed models in nmle package?

I am running mixed models analyses because I have participants nested in groups. Across the different analyses, I have very different degrees of freedom in the lme output with nlme package (and...

09 February 2021 6,767 3 View

Which software is the best to model a non-linear concrete (ABAQUS, ANSYS,ADINA, ATENA, etc.)?

What is the best finite element modeling software packages for modeling non-linear and fracture analysis of CONCRETE? I've had some with ABAQUS standart/explicit, but I would like to learn if...

27 January 2021 330 6 View

How plot results of membership function in R?

I am using the FRBS package, I developed the model without learning data and I use the plotMF function of this package to see the membership function of every input and output variables. I got the...

22 January 2021 2,668 3 View