Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

What is the best way to extract Spike protein from whole cell lysates for ELISA ?

Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...

17 September 2020 7,265 4 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

How to associate parameters to function switch?

I am using comsol to calculate the IV curve using a physical model. The problem is that I am using the switch function to evaluate multiple spectra but I can not associate a temperature for each...

30 June 2019 2,894 1 View

Why in the simulation of a fracture toughness test, the values of stress intensity factor are not uniform along the crack of the specimen?

In the virtual simulation of toughness using a standard steel specimen (A516-Gr.70), the maximum equivalent (Von-Mises) stresses are uniform along the entire length of the crack. However, when the...

21 January 2019 3,694 1 View

Does anyone know an alternative co-stimulation signal to feeder cells or soluble CD28 for CD4 T cells?

I want to evaluate the CD28 loss on CD4 T cells in culture with different stimuli. I used to stimulate this cells with anti CD3/CD28 dynabeads but actually i don't know if it could interfere with...

31 December 2018 2,629 3 View

Can someone tell me where can I find the theory of anxiety postulated by Zung or the original manual?

I need it to write my thesis on anxiety and maternal attitudes in mothers in prision.

11 September 2018 9,452 1 View

How to design qPCR primers specific to cDNA in two different species (mouse & humans)?

Neither my supervisor or I have done qPCR for a number of years and he asked me to look into designing qPCR primers for detecting UCP1 expression in both humans and mice. We would prefer to use a...

10 September 2018 7,542 3 View

How can I understand a SEM micrograph?

Hi, I am trying to identify what phases are present in these pictures, can someone help where can I learn about it? I will really appreciate if someone can give me comments about these pictures.

31 July 2018 3,682 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View