What is the damping coefficient of Well's/impulse turbine that may be introduced into a mechanical oscillation model to simulate the PTO damping?

In tank experiments, it is easy to simulate the PTO damping by the use of orifices, however in modeling full scale and real conditions the trurbine damping coefficient must be added to the water...

31 January 2021 7,325 1 View

I do not want to be pessimistic, but can a pandemic emerge that has no cure and also has no diagnostic methods ??

It was our good luck that a diagnosis of this pandemic could have been made, but could a new epidemic emerge without a diagnosis or treatment ?? Will we be ready for it? I think this is one of the...

29 May 2020 5,787 12 View

How I can know the knocking out effect of specific target sequence by the CRISPR/Cas9 using RT-PCR?

I am doing knocking out for specific sequence by pX458 plasmid and I already did qPCR after 72h of transfection, I have used pX458 plasmid without gRNA as negative control but it seems that has...

19 December 2018 5,676 2 View

ANSYS Divergence problem?

How can i solve this error Error: Divergence detected in AMG solver: pressure correction Error Object: #f ?

14 September 2018 9,210 3 View

How to determine the loss of function of a protein in vitro?

I am trying to find the best method to determine the effects of adding an inhibitor on the function of one protein ( transmembrane) in cell culture. Thank you!

15 August 2018 7,737 5 View

What is the main ecological impacts of climatic changes ?

ecological impacts

25 March 2018 7,272 9 View

Do capacitors produce harmonics when connected at the load side? and if yes, why?

18 September 2014 4,016 11 View

In MATLAB/SIMULINK, is there a rule to follow when selecting the solver which will provide the most accurate results?

For example when one wants to simulate an electrical circuit using SimPowerSystems.

03 June 2014 7,849 3 View

How can I simulate SVC, STATCOM, TCSC, SSSC, and UPFC connected in a network (as simple as possible) using MATLAB/SIMULINK?

Network flows and voltage control simulation using MATLAB/SIMULINK.

08 May 2014 3,590 4 View

About the fair of citation rate

What is your vision about the justice of the citation? As it is possible to publish articles in some scientific fields, where there is no many scientific works because of the lack of scientists...

01 January 1970 248 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View