As I am trying to pipette a liquid solution from 25 ml solution into 25 different tube but each time my solution ran out before filling all those tubes. Now what should I do?
… prepare more solution!
Pipettes are usually calibrated for outflow (There is probably a lable on your pipette that reads “25 ml ex”.), and this means that a drop of liquid is expected to remain in the pipette. In your case, you need about 25.03 ml to fill a dry pipette, and 25.00 ml will flow out when you empty it.
The volume of the remaining droplet depends on the surface tension of the liquid, temperature, and other factors, of course. Pipettes are not meant for really accurate work.
When I pipette from a master mix solution into different tubes I usually add around 20% to the master mix total volume to have enough liquid.
In example if I need 10 mcL in 5 separate tubes I don't make a mix of a total of 50 mcL, I make a 60 mcL mix to accommodate the liquid that will be left in the pipette tips.
The pipette must be standardised . Then use a vacuum device to suck so that
the miniscus must be accute at the eye level.
I would recommend a small lab device called Pipette Genie. It was really helpful when dealing with a lot of samples on 384 well microplate. Here is their website, if it helps.
https://www.pipette-genie.com/
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