I am looking at cFos activation in the brain, and have a few areas of interest. I am also looking at left/right lateralisation in each area.
I have 3 groups (naive, vehicle, test) with uneven sample sizes. For each animal I have an average value per area, left and right hemispheres (cells/area).
I am thinking I should do a 2-Way ANOVA (using group and left/right as factors), but the software I use (OriginPro) won't let me do this analysis with different group sizes.
Any thoughts?
You presented a correct approach using two-way ANOVA (with repeated measures on the "side" factor). If your software do not allow it, change the software! SPSS can perform it (assuming that you have the same number of measures on the repeated measures factor). I prefer to use R, but is not so easy to beginners...
Don't forget to use the appropriate post-hoc test (I probably would use Bonferroni or Tukey-Kramer), given the different sample sizes.
Use SAS:
PROC GLM DATA=yourdata;
class group left_right;
model average_value=group left_right;
run;
You simply need to replace the bold characters with your real variable names. Let me know!
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