Estimate Beta is .00, yet it's significant?

Hi everyone, I've run an analysis of a latent 3-way interaction in Mplus (Y on XWZ). The estimate for this path is 0.000. S.E. is 0.000. Est./S.E. is 2.034. Two tailed p-value is 0.042. So,...

08 December 2020 2,132 4 View

What is the double layer capacitance of a 1 sq-cm smooth gold electrode in 0.1N (0.05M) Sulfuric Acid?

Is there an empirical or theoretical standard value for this (at room temperature) and for different electrolyte concentrations? Is there a resource available that provides a chart of values for...

29 November 2020 6,937 2 View

How does the concentration of atmospheric oxygen near ground level vary with time?

Hello, I am interested in how the concentration of O2 varies over time close to ground level. I have been informed that the concentration can drop when an inversion layer is present in the local...

19 November 2020 2,328 3 View

How does one calculate the best volume of nickel gel to use in a protein purification?

We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...

15 November 2020 4,664 2 View

Final temperature of water leaving an ice bath through copper tubing?

I have water flowing at 4 GPM at 2.5 feet per second through 50ft of 0.375 in. outer diameter tubing with 0.014 in. wall thickness. The water flows through copper tubing into the 50ft coil...

01 November 2020 8,039 5 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

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03 October 2020 4,471 3 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

13 July 2020 1,773 3 View

Where to find predator:prey mass data (for terrestrial carnivores)?

I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds). An example of is...

16 June 2020 2,453 9 View

How to calculate the overlap between animal distributions (Shape or .shp file)?

I would like to calculate the overlap between the distributions of two sets of data (predator & prey). The prey set has got about 250 species, the predator set approximately 5000. For each...

21 May 2020 6,726 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View