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Fundacao Casa de Rui Barbosa
www.casaruibarbosa.gov.br
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...
03 June 2024 6,221 1 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
28 May 2024 3,529 1 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...
27 May 2024 5,013 1 View
Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...
21 May 2024 2,171 0 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...
12 May 2024 1,045 4 View
May I request for change of pofile for this email address? It belongs to the ofice of the head of institution but the name attached is different. Kindly also include [email protected] being the...
17 July 2024 1,145 0 View
There are few business activities more prone to a credibility gap than the way in which executives approach organizational life. A sense of disbelief occurs when managers purport to make decisions...
08 July 2024 1,323 2 View
How do I correct my institution of affiliation?
28 June 2024 5,526 1 View
Dear fellows, yesterday, when trying to register another reference in EndNote, I got the message that the library was full. It has more than 5000 references. I wonder thus, what is the smartest...
25 June 2024 6,903 1 View
Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...
24 June 2024 1,242 0 View
My friend who is a Credit Review Officer is working on a research project. The outcome need to be published hence facing difficulties. Bank of Bhutan is not allowed in institution details. Any...
06 June 2024 990 2 View
I had a valid idea for a meta-analysis involving multiple prospective & retrospective studies with no prior meta-analyses on the same chosen sub-population. When confirming the validity of my...
04 June 2024 8,682 1 View
One of my friends could not register an account on Researchgate with his own email (like gmail). Should we register an account with the school email?
31 May 2024 6,347 3 View
need paper of IIT khargaur and energy IIT mumbai
26 May 2024 4,316 1 View
Hello, This is my VERY first time attempting a NEB calculation, and I must admit, I'm feeling quite confused. I would sincerely appreciate any feedback or suggestions. Here's the...
07 May 2024 5,745 9 View