23 April 2025 0 10K Report

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Fundacao Casa de Rui Barbosa

www.casaruibarbosa.gov.br

More Ana Maria Pessoa dos Santos's questions See All
What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,807 3 View

A variable moderating multiple relation - how can I test hypotheses using amos?

How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...

03 June 2024 6,221 1 View

Any experience in fibroblast centrifugation?

We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...

28 May 2024 3,529 1 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

Are the calibration curves of NO radical scavenging assay asymtotes?

Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...

27 May 2024 5,013 1 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

12 May 2024 1,045 4 View

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Identity behind this address?

May I request for change of pofile for this email address? It belongs to the ofice of the head of institution but the name attached is different. Kindly also include [email protected] being the...

17 July 2024 1,145 0 View

Given the organizational complexity of academic institutions does an internal institutional politics play significant role in an institution's growth?

There are few business activities more prone to a credibility gap than the way in which executives approach organizational life. A sense of disbelief occurs when managers purport to make decisions...

08 July 2024 1,323 2 View

How do I correct my institution of affiliation?

How do I correct my institution of affiliation?

28 June 2024 5,526 1 View

What to do when you have reached the treshold of references in EndNote?

Dear fellows, yesterday, when trying to register another reference in EndNote, I got the message that the library was full. It has more than 5000 references. I wonder thus, what is the smartest...

25 June 2024 6,903 1 View

How can we generate topology file for water and helium system?

Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...

24 June 2024 1,242 0 View

Can a banker or practicing professional allowed to create an ORCID account ?

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06 June 2024 990 2 View

What to do if your idea for a meta-analysis is already registered on PROSPERO since 2.5 yrs by others but no research outcome has been produced yet?

I had a valid idea for a meta-analysis involving multiple prospective & retrospective studies with no prior meta-analyses on the same chosen sub-population. When confirming the validity of my...

04 June 2024 8,682 1 View

Can we register researchgate account by personal email?

One of my friends could not register an account on Researchgate with his own email (like gmail). Should we register an account with the school email?

31 May 2024 6,347 3 View

EPI of educational institution of india?

need paper of IIT khargaur and energy IIT mumbai

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NEB Calculation Issue?

Hello, This is my VERY first time attempting a NEB calculation, and I must admit, I'm feeling quite confused. I would sincerely appreciate any feedback or suggestions. Here's the...

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