Performing in silico CRISPR Cas docking involves the use of computational methods to predict the interaction between the Cas protein and the guide RNA (gRNA). Here are some general steps that can be followed for in silico CRISPR Cas docking:

Designing a gRNA along with a tDNA for any Cas9 protein involves selecting a target site in the genome and designing a gRNA that will guide the Cas9 protein to that site. Here are some general steps that can be followed for gRNA design:

There are several tools available for designing gRNAs and predicting their efficacy. Here are some commonly used ones:

Note that there are many other tools available, and the choice of tool will depend on the specific needs of the user and the system being studied.