How nutrients move from mycorrhizal fungi to root cells?

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How to relate relaxation time values obtained from different equation?

I tried to fit the frequency vs Dielectric constant and f vs Dielectric Loss graph with HN equation. I got different Tau (relaxation time) values from the fittings of both graphs. What I have to...

12 January 2021 8,467 6 View

Can anybody tell me about the basic principle behind the use of neutral and alkaline comet assay ?

In most of the research paper people used both neutral and alkaline comet assay to determine the level of DNA damage either single stranded or double stranded but the exact principle of both the...

02 August 2020 2,193 2 View

Is it possible to provide online education to all the children studying in the schools during this lockdown period?

In many countries, the children belong to remote villages and do not have facility of computer or Android mobile and internet connectivity. Do you think, the countries will be able provide online...

17 May 2020 2,739 16 View

DFT calculation of Diradical Singlet in Turbomole?

Using TURBOMOLE, I am studying a mechanism of Heterolytic vs Homolytic cleavage. When the bond breaks, there are three possibilities a) Heterolytic Cleavage (RHF, singlet) b) Homolytic Cleavage...

15 October 2019 4,214 5 View

What is a standard method to isolate and compare bioactive peptides in milk sample?

Need methods for isolation and characterization of bio-active peptides in milk with proper protocols for the same.

12 December 2018 3,818 2 View

How to obtain the MODIS vegetation index for a particular date which lies within the 16 day composite ?

I want to obtain the vegetation index map for a date which lies in between the 16 day composite MODIS vegetation indices. Ex- Let's say the MODIS (both terra and aqua) has 16 day composite images...

01 September 2018 9,489 6 View

Which predictive model would be the best one to design a scoring model for Big data?

Logistic regression, SVM, andom forest etc

24 July 2018 1,019 4 View

What could be a suitable jet polishing solution and parameters that I can use for TEM studies of precipitates in 718Plus?

I have come across a lot of journals with different solutions. But I am not sure which one can be used. Please suggest.

19 July 2018 1,903 1 View

How increase in flexural strength can be compared with decrease in flexural modulus ?

In detail, I performed flexural test of some composite specimen. I got higher flexural strength with high modulus. But after doing some modification in main material, I got enhanced flexural...

15 April 2018 4,826 7 View

Could anyone suggest some articles/ reviews which show MHC II expression of murine monocytes and macrophages in different tissues during inflammation?

I am looking into monocytes and macrophage in murine model (C57Bl/6) of a disease.

22 March 2018 959 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View