How multivariants data can be analysed?

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How miRNA NGS abundance correlates with signal intensity in in situ hybridisation?

We performed next generation sequencing of miRNAs and then did in situ hybridisation of some of the identified miRNAs. The relative in situ signal strength and read number between some miRNAs...

08 September 2017 4,582 3 View

What would be the best representation of many data?

Hello everyone, I have data for 20 amino acids, at four time points in each of three varieties. What would be best type of graph to display them properly, possibly in one single graph. Any...

03 April 2017 8,559 1 View

What would be the best for miRNA NGS validation?

I would like to validate miRNA NGS data by qRT-PCR. This theoretically could be done from total RNA, isolated small RNA (since that was used for sequencing library preparation) or the sequencing...

10 November 2015 9,598 4 View

Has anyone an idea why no impact factor is shown for MGG publications?

I have some uploaded publications that appeared in MGG long time ago, and no impact factor is appeared for them. Perhaps ResearchGate admin should answer this, but any suggestion is welcomed.

03 April 2015 1,122 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Which statistical test should we use?

N=6 Comparing pre and post test likert scale responses. Participants are mix of practicing & preservice teachers.

30 July 2024 7,233 4 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

25 July 2024 8,108 0 View

Is a reliability test necessary in my survey on translations?

Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...

24 July 2024 8,245 5 View

Best statistical test for three groups and binary dependent variable ?

Hello everyone, I am currently working on a project where I aim to analyze color preferences in data visualizations across participants from three different countries. The dependent variable in...

19 July 2024 4,178 2 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View