Sayoni Dutta Hi..

You need for each bacterial type and each sample , 10 ml × 5 for each double concentration tube , 1 ml × 5 for each simple concentration tube and 50 ml for the bottle.

Hope this help you .

A lot will depend on your ultimate goals and constraints. What you might sample or enrich for a class experiment would be different for thesis, dissertation or recognized published paper on the subject. Backwashing concentration may be exceedingly high at first and taper off with time. Do all bacteria release easily from media in backflushing?, or is there variability with time on release. Will you be grabbing sample, or have some type of automated sampling, single individual samples or composite sampling? Probably best to put samples on ice for transport with testing within one day based on Stanard are methods Standard Methods. (IPad has typing issues). I don’t have all the technical expertise to answer all possible questions. If sampling sewage treatment residues, you might consult someone about potential for COVID-19 and act accordingly. Write up a study plan and have your professor, statistician or microbiologist review and comment. You don’t want to do too little planning and sampling only to find out it was inadequate, unless you are doing some introductory sampling and running unbind experiments to then develop a more informed study or research plan. (Remove the word unbind ipad issues)

100 ml water sample require for bacteriological analysis.

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You need for each bacterial type and each sample , 10 ml × 5 for each double concentration tube , 1 ml × 5 for each simple concentration tube and 50 ml for the bottle.